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KMID : 0903520040470040426
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 4 p.426 ~ p.429
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Development of Biologically Active Compounds from Edible Plant Sources-XI. Analysis of Phytol Content in Several Varieties of Lactuca sativa L. by gas chromatography
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Song Myoung-Chong
Lee Jin-Hee
Bang Myun-Ho
Kim Ji-Young
Chung Dae-Kyun
Pai Tong-Kun
Kim In-Ho
Baek Nam-In
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Abstract
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An easy, sensitive and rapid method for the analysis of phytol, a strong ACAT (Acyl-CoA: Cholesterol Acyltransferase) inhibitory diterpenoid, was established. The pre-treatment of sample for GC analysis of phytol includes aq. MeOH extraction, partition of the extracts between EtOAc and water, and filtration of organic phase through Supelclean LC-Si SPE tube. The GC analysis of phytol for a variety of Lactuca sativa was carried out on DB-5 capillary column with flame ionization detector (FID). The consistency in phytol contents according to each variety was not shown. Especially, Jeoktoma, as RS-2 type, showed the highest phytol content.
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KEYWORD
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Lactuca sativa L., phytol, GC
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